As Associate Scientist within the Molecular Analytics platform, you will be in charge of implementing, designing and developing methods for the detection and quantification of nucleic acids based on up-to-date nucleic acid technologies (NAT) and, in particular, methods aiming at characterizing plasmids (topology, conformation, sequence, quantity, etc.
mRNA Drug Substances (content, integrity, identity) and mRNA-LNP Drug products (monovalent and multivalent), used for production of mRNA vaccines.
The use of direct nucleic acid quantification methods (e.g. CRISPR-based assays, digital counting through optical barcodes, branched DNA assays, etc.
will part of the activities of the hired Scientist.
You will join the Analytical Development Department, where the Molecular Analytics Platform is in charge of developing modern, state-of-the-art molecular methods to analyze (i.
e. detect, identify, quantify and characterize) nucleic acids at all stages of vaccines development.
You will be in charge of the design and development of NAT-based analytical methods to support product and process development, to develop and transfer release methods for GMP testing for Phase 1, Phase 2 and Phase 3, according to Quality by Design principles and in partnership with Technical Research and Development functions, Quality and Regulatory.
Work on the bench in the labs (>
Interact with Belgium- and US-based team members
Contribute to the development, qualification, validation and transfer of innovative genetic characterization assays / technologies based on state-of-the-art NAT
Ensure the development of analytical methods for purity, identity and sterility
Execute analytical methods for the genetic characterization of genetically engineered microorganisms, including recombinant plasmids
Interact and act in a complex matrix with several stakeholders from different departments (Research, Development, QC, RA, QA)
Interact with other GSK Vaccines R&D Centers (in US and IT) and external CMOs
Ensure implementation of QbD (Quality by design) in the development of assays
Agile principles driven
Develop detailed experimentation protocols and executes the planning / organization of the experiments in his / her scope of activities
Analysis and interpretation of the data, including reporting and presentation
Contribute to the various activities of the group
Basic Qualifications :
We are looking for professionals with these required skills to achieve our goals :
Bachelor / Master degree (biotechnology, molecular biology, biochemistry, (molecular) bacteriology, (molecular) microbiology ) with 3 to 5 years of experience in the industry (in biologics / biopharmaceuticals / pharmaceutics) or equivalent by experience
Experience in a lab environment focusing on analytics of biomolecules
Diversified expertise in molecular biology (diversity of techniques and technologies as well as diversity of subjects)
Nucleic acid extraction
PCR, QPCR and ddPCR
Direct nucleic acid quantification methods (e.g. CRISPR-based assays, digital counting through optical barcodes, branched DNA assays, etc.)
Preferred Qualifications :
If you have the following characteristics, it would be a plus :
Bacteriology, microbiology techniques, including plasmid biology
Sanger and high throughput sequencing technologies
Design of primers and probes using recognized software's, design of LNA-containing primers and probes, design probes for multiplex assays, design of taqman probes,
Molecular Biology techniques (including Southern blot, restrictions, reverse transcription, in vitro transcription, electrophoresis, etc.)
Ability to conduct literature and online research, continuous search of new methods
Identification of critical method parameters and plan to evaluate them
Good writing skills
Attention for details, eager to learn and driven by curiosity